The objective of this Diploma Thesis is to investigate the impact of mobility characteristics on road safety in European cities. For this analysis, various international databases were exploited with data on road accident fatalities, demographics and mobility characteristics of 25 European cities in 2012. Generalized Linear Models were developed for both the total number of fatalities and for specific subcategories too. The results led to the conclusion that more public transport capacity offered, more cycle trips and fewer motorcycles lead to a reduction in the number of fatalities in urban road accidents. Moreover, it was found that denser road network, higher population density and higher GDP per capita are correlated with fewer fatalities in urban road accidents.