The aim of the present research is to investigate the attitudes of users for sustainable mobility in Mediterranean Universities, so that the necessary conclusions can be drawn for the disposal of the passengers in this direction as well as the criteria that affect their implementation. The survey was conducted electronically and on site via a questionnaire, which was completed exclusively by members of 7 Mediterranean university communities. The data gathered were analysed through factor analysis and two linear regression model were developed regarding the preferences of the respondents for the various means of transport, their attitude to the mobility problems, as well as to the proposed measures of improving the traffic conditions. Results indicate the importance of several parameters, such as travel time, security and flexibility, comfort, cost and reliability.