The performance of Road Safety Audits (RSAs), either in the design or rehabilitation of new infrastructure projects, or for assessing the safety level of existing roads (RSIs), has great potential to improve transport safety and reduce the both crash rates and crash severity indices. The scope of the present study is to review and provide insights on the international experience on the implementation of RSAs, focusing on countries that are pioneers in this field and have established clear and robust procedures. The present study is based on a questionnaire survey addressed to road safety experts from several countries, along with a comprehensive review of RSA guidelines and manuals on the procedures followed in the UK, Australia, USA, European countries, as well as Gulf States. Moreover, European Commission Directives 2008/96/EC and 2019/1936/EC on road infrastructure safety management are also examined. The study presents interesting combined findings from both the questionnaire survey and the review of guidelines in order to provide an overall synthesis of international experience in Road Safety Audit implementation, leading to the identification of related best practices. The paper aims to introduce RSA procedure in countries where it is not applied and improve the existing RSA procedures.