The autonomous vehicles are expected to bring unprecedented changes in the labor sector and the workforce. Traditional jobs will be alleviated, new will be created while people involved in the autonomous vehicle operation should be qualified with additional skills and knowledge in order to be able to deal with the new technology and the various systems. Furthermore, the impact on the role of the ‘driver’ is anticipated to be significant in all transportation modes. The purpose of the present research is to identify the skills and knowledge required for an efficient and proper operation of any autonomous vehicle. Both professional and private operators and all transportation sectors (road, rail, maritime, aviation) and autonomous levels will be considered as each one has different requirements.