The objective of this Diploma Thesis is the multilevel analysis of road accident characteristics in urban areas in Europe. For this analysis a database was developed containing, road fatalities, characteristics of the driver, the road environment and the vehicle, GDP per capita, population in urban areas and vehicle fleet for 24 European countries for the year 2009. Simple and multilevel models were developed and applied. The results led to the conclusion that the use of hierarchical data structures and nested data structures, for data concerning accident in urban areas of Europe is necessary for the correct estimation of the parameters impact. From the second level analysis a differentiation in the number of fatalities in the various European urban areas was found, depending on the specific characteristics of these areas. Moreover the application of these models led to the identification of the impact of various parameters such as weather conditions, vehicle type, fatality age on the number of fatalities in the various urban areas of the European countries.