The objective of this research is to investigate the effect of advertising signs on the number of road accidents by means of statistical methods. On that purpose a statistical analysis using before-and-after techniques with large and small control groups was carried out in 9 different road axes within the greater Athens area, in which placement or removal of advertising signs took place during the last decade. From the statistical analysis of the road axes examined it is shown that no statistically significant correlation between road accidents and advertising signs can be established in none of the cases examined.