The objective of the present study is to investigate the preferences towards e-scooters in Athens and to identify the most important factors that affect the choice of the Athenians towards means of transport. To collect the required data, a questionnaire-based stated preference survey was carried out, including hypothetical scenarios of cost, time and comfort, answered by 202 Athenian travellers. Afterwards, logistic regression models were developed (multinomial and binary), from which the utility factions, that mathematically describe the behavior of the Athenians towards e-scooters, were extracted. The results showed that the probability of choosing an e-scooter depends largely on the cost, time, comfort, views, habits and the demographic characteristics of the respondents. The faster and the more economical the trip and the more familiar the respondent with e-scooters is, the more likely they are to choose them over other means of transport.