A NTUA Diploma Thesis titled “The impact of physical fitness on road safety and driver behaviour on rural roads” was recently presented by Marina Karachalia. For this reason an experimental procedure involving 46 participants aged 19–27 was conducted. The participants were evaluated in terms of their cardiorespiratory fitness through the Maximal Oxygen Uptake index (VO₂max), while also  completing a related questionnaire. VO₂max was calculated based on heart rate (BPM – Beats Per Minute) after recovery from a short physical activity and then were categorized into two fitness groups: high fitness and low fitness, based on established VO₂max classification thresholds. The data was analyzed using linear and logistic regression models. Results suggest that high-fitness drivers have longer reaction times, greater variability in headway distance, higher average speeds, and, conversely, a lower probability of road accident involvement. Overall, higher physical fitness appears to be associated with more stable and controlled driving behavior, which, despite the increased reaction time, may contribute to reduced accident risk through improved decision-making.