The Department of Civil, Environmental, Architectural Engineering and Mathematics of Universita Degli Studi di Brescia, organised with great success the 25th Living and Walking in Cities Conference in Brescia, on 9-10 September 2021. The LWC International Conference traditionally deals with the topics of urban mobility and quality of life in urban areas, with a specific focus on vulnerable road users. The main areas and topics of the Conference were transport system and infrastructure, safe mobility for innovative cities and urban planning NTUA contributed actively with the following presentation: